연구 및 대학원

연구 및 대학원

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대학원 세미나

05/21(목)16시 연사: 장혜식 (서울대 생명공학과 교수)
작성자 관리자조회수 69날짜 2020.05.19

일시: 05/21(목)16시

장소: 온라인 세미나(ZOOM)로 진행 ▶https://snu-ac-kr.zoom.us/j/94542994614 (Meeting ID: 945 4299 4614)

강사: 장혜식(서울대 생명공학과 교수)

제목: Direct RNA sequencing reveals transcript-specific RNA modifications in the SARS-CoV-2 transcriptome

내용: SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3′ tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.

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